Junyi JY-SCZ6 Vertical Electrophoresis Tank

Overview

The Junyi JY-SCZ6 Vertical Electrophoresis Tank is an engineered platform for high-fidelity SDS-PAGE, native PAGE, and two-dimensional electrophoresis applications in molecular biology and protein biochemistry laboratories. Designed around the fundamental principles of constant-voltage or constant-current electrophoretic separation in polyacrylamide matrices, the JY-SCZ6 supports reproducible migration of proteins and nucleic acids under controlled electric field conditions. Its vertical configuration enables precise stacking and resolving gel assembly, minimizing band distortion caused by lateral field inhomogeneity—a critical factor in achieving sharp, vertically aligned electrophoretic bands. The system operates without integrated power supply (requiring external DC power source), allowing full compatibility with programmable electrophoresis power supplies meeting IEC 61010-1 safety standards.

Key Features

• Edge-free electric field geometry: Precision-machined acrylic chamber and electrode alignment eliminate fringe field effects at gel peripheries, significantly improving band resolution and straightness—especially critical for quantitative densitometry and downstream Western blotting.
• Lid-activated safety interlock: Integrated mechanical switch interrupts power delivery upon lid opening, complying with laboratory electrical safety protocols for Class I equipment.
• Modular electrode assembly: Platinum-coated stainless-steel electrodes are mounted on detachable carriers, enabling rapid cleaning, calibration verification, or replacement without disassembling the main tank body.
• Dual-thickness gel compatibility: Accommodates standard 1.0 mm and 1.5 mm gel cassettes; each thickness supports interchangeable 21- and 35-well comb configurations for flexible sample throughput planning.
• Seamless gel casting integration: Optional gel-casting stand and proprietary sealing strips allow independent gel polymerization outside the electrophoresis run chamber—reducing cross-contamination risk and enabling parallel preparation workflows.
• Compact footprint and ergonomic access: Overall dimensions (263 × 145 × 220 mm) optimize benchtop space utilization while maintaining unobstructed front-panel access for buffer filling, comb insertion, and gel loading.

Sample Compatibility & Compliance

The JY-SCZ6 is validated for use with standard acrylamide/bis-acrylamide formulations (e.g., 8–20% T), Tris-Glycine, Tris-Tricine, and Bis-Tris SDS-PAGE buffers. It supports common precast and hand-cast gels, including gradient and gradient-stabilized formats. The system meets general laboratory safety requirements per GB/T 14710 (Chinese national standard for environmental adaptability of medical electrical equipment) and conforms to structural design expectations outlined in ISO 13485:2016 Annex C for ancillary life science instrumentation. While not a regulated medical device, its mechanical safety architecture—including insulated wiring, non-corrosive chamber materials, and fail-safe lid interlock—is aligned with GLP-compliant lab infrastructure expectations.

Software & Data Management

As a standalone electrophoresis tank, the JY-SCZ6 does not incorporate embedded firmware or digital control interfaces. All operational parameters—including voltage, current, and run duration—are managed externally via compatible power supplies with data logging capabilities (e.g., Bio-Rad PowerPac HC, Thermo Fisher Ettan™ EPS 3501 XL). When paired with CCD-based gel documentation systems (e.g., Azure Biosystems Sapphire, Syngene G:BOX), raw electropherograms can be imported into open-format analysis platforms such as ImageLab™, Fiji/ImageJ, or AlphaView SA for band quantification, molecular weight estimation, and comparative expression profiling. Audit-trail generation depends entirely on the connected power supply and imaging system’s compliance with FDA 21 CFR Part 11 requirements.

Applications

• High-resolution separation of denatured proteins (10–250 kDa) in SDS-PAGE for purity assessment and QC release testing.
• Native electrophoresis of multi-subunit complexes, enzymes, and membrane proteins under non-denaturing conditions.
• Preparative-scale electrophoresis for excision and extraction of target bands prior to mass spectrometry identification.
• First-dimension immobilized pH gradient (IPG) strip handling in 2D-PAGE workflows when used with compatible focusing trays.
• Teaching laboratory implementation: Robust mechanical design and intuitive assembly support undergraduate and graduate-level instruction in electrophoretic theory and technique.

FAQ

Does the JY-SCZ6 include a built-in power supply?

No. It is a passive electrophoresis tank requiring connection to an external DC power supply capable of delivering up to 500 V and 500 mA.
Can it accommodate precast gels from major vendors?

Yes—standard 160 × 180 mm precast gels (e.g., Bio-Rad Criterion™, Thermo Fisher Novex™) fit directly into the cassette holder with minor adapter adjustments.
Is the tank compatible with silver staining and fluorescent dyes?

Yes. The transparent acrylic chamber allows full optical access for post-electrophoresis visualization using UV transilluminators, blue-light scanners, or chemiluminescence imagers.
What maintenance is required for long-term reliability?

Rinse thoroughly with deionized water after each use; inspect electrode surfaces quarterly for platinum coating integrity; store upright with lid secured to prevent dust ingress into buffer chambers.
Are spare parts such as combs and sealing strips available separately?

Yes—Junyi provides OEM-compatible consumables including 21-well and 35-well combs (1.0 mm and 1.5 mm), gel sealing strips, and replacement electrode assemblies through authorized distributors.