Junyi JY-SPGT Horizontal Electrophoresis Tank
| Brand | Junyi |
|---|---|
| Origin | Beijing, China |
| Model | JY-SPGT |
| Instrument Type | Nucleic Acid Electrophoresis Apparatus |
| Gel Dimensions (W×L) | 200×200 mm, 200×150 mm, 200×100 mm |
| Sample Capacity | 264 wells |
| Buffer Volume | ~1800 mL |
| External Dimensions (L×W×H) | 397×230×93 mm |
| Net Weight | 3.3 kg |
| Gel Tray Handle Design | Yes |
| Safety Interlock | Automatic Power Cut-off Upon Lid Opening |
| Electrode Assembly | Removable |
| Gel Casting System | Pre-sterilized, No-Prep Comb Included |
| Leveling Adjustment | Integrated Adjustable Feet |
| Material | High-Transparency Imported Polycarbonate (PC), One-Piece Injection Molded |
Overview
The Junyi JY-SPGT Horizontal Electrophoresis Tank is an engineered platform for routine and high-throughput nucleic acid separation in molecular biology laboratories. Designed according to standard horizontal electrophoresis principles—where DNA fragments migrate through a porous gel matrix (typically agarose or polyacrylamide) under the influence of a uniform electric field—the JY-SPGT enables reliable size-based resolution of DNA fragments ranging from ~50 bp to >20 kbp. Its modular architecture supports interchangeable gel trays and comb configurations, facilitating flexibility across experimental scales—from small-scale verification runs to large-format screening applications. The system operates without integrated power supply (external DC power source required), maintaining compatibility with standard laboratory electrophoresis power supplies meeting output specifications of 0–300 V, 0–500 mA.
Key Features
- High-transparency imported polycarbonate construction ensures optical clarity for real-time gel monitoring and post-run UV visualization, while providing mechanical rigidity and chemical resistance to common electrophoresis buffers (TAE, TBE, SB).
- Integrated safety interlock mechanism automatically interrupts electrical continuity when the lid is opened—complying with IEC 61010-1 requirements for laboratory electrical equipment safety.
- Ergonomic tray design includes dual molded handles for secure, slip-resistant handling during gel pouring, loading, and transfer—reducing risk of buffer spillage or gel fracture.
- Adjustable leveling feet enable precise horizontal alignment on non-uniform lab benches, ensuring uniform electric field distribution and minimizing band distortion.
- Removable electrode assembly simplifies cleaning, inspection, and replacement—supporting long-term reproducibility and reducing downtime during routine maintenance.
- Includes pre-calibrated, no-prep combs available in multiple tooth configurations (17-, 22-, 36-, and 44-well) for both 1.0 mm and 1.8 mm gel thicknesses—eliminating comb alignment errors and buffer leakage during polymerization.
Sample Compatibility & Compliance
The JY-SPGT accommodates standard agarose and native/polyacrylamide gels cast in three fixed dimensions: 200 × 200 mm, 200 × 150 mm, and 200 × 100 mm. Each configuration supports up to 264 sample wells at maximum density (44-well comb × 6 rows), enabling parallel analysis of multiple samples under identical electrophoretic conditions. The tank’s 1800 mL buffer reservoir maintains stable pH and ionic strength over extended runs (up to 120 min at 120 V). All materials are certified free of DNase/RNase activity per ISO 13485-compliant manufacturing protocols. The device meets general laboratory safety standards aligned with GLP documentation practices and supports audit-ready workflows when paired with validated power supplies and imaging systems.
Software & Data Management
As a benchtop electrophoresis platform, the JY-SPGT does not incorporate embedded firmware or digital control interfaces. It functions as a passive, analog separation module compatible with third-party documentation systems—including gel documentation stations (e.g., Bio-Rad GelDoc, Azure Biosystems Sapphire) and image analysis software (ImageLab, Quantity One, Fiji/ImageJ). Users may log run parameters (voltage, time, gel concentration, buffer type) manually or via LIMS-integrated electronic lab notebooks (ELNs) compliant with 21 CFR Part 11 when configured with appropriate access controls and audit trail functionality.
Applications
- Verification of PCR amplification products and restriction digestion patterns.
- Estimation of DNA fragment size using calibrated ladders (e.g., GeneRuler 1 kb DNA Ladder, Lambda HindIII digest).
- Quality assessment of plasmid preps, genomic DNA extractions, and NGS library preparations.
- Separation and recovery of DNA bands for downstream cloning, sequencing, or enzymatic modification.
- Teaching laboratory implementation of core molecular techniques in undergraduate and graduate curricula.
FAQ
Does the JY-SPGT include a built-in power supply?
No. The unit requires an external DC power supply capable of delivering 0–300 V and 0–500 mA.
Can I use this system for SDS-PAGE or protein electrophoresis?
Not recommended. The JY-SPGT is optimized for nucleic acid separation in neutral or slightly alkaline buffers; it lacks cooling capacity and electrode geometry required for denaturing protein electrophoresis.
Is the gel tray autoclavable?
The polycarbonate tray is heat-resistant up to 121°C for short durations, but repeated autoclaving may compromise optical clarity and structural integrity; chemical sterilization (e.g., 70% ethanol) is preferred.
What buffer volumes are supported across different gel sizes?
All configurations operate within a nominal 1800 mL buffer volume range; minimum working volume is 1200 mL to ensure full submersion of the gel surface and stable current distribution.
Are replacement combs and electrodes available separately?
Yes. Junyi offers OEM-specified spare parts including 1.0 mm / 1.8 mm combs (17/22/36/44-well), platinum-coated stainless-steel electrodes, and sealing cassettes—available through authorized distributors with traceable lot documentation.
