Junyi JY-SPHT Horizontal Electrophoresis Chamber

Overview

The Junyi JY-SPHT Horizontal Electrophoresis Chamber is a precision-engineered platform for nucleic acid separation and molecular weight estimation under standard agarose or polyacrylamide gel electrophoresis (AGE/PAGE) protocols. Designed in strict adherence to the physical principles of horizontal electrophoresis—where charged biomolecules migrate through a porous matrix under a uniform electric field—the JY-SPHT supports reproducible DNA fragment resolution across common analytical ranges (50 bp–20 kbp). Its open-tank architecture facilitates rapid gel casting, post-run staining, and UV visualization without disassembly, making it particularly suitable for routine molecular biology laboratories conducting genotyping, PCR product verification, restriction digest analysis, and plasmid QC.

Key Features

• Monolithic polycarbonate construction ensures optical clarity (>92% transmittance at 254 nm), chemical resistance to common electrophoresis buffers (TAE, TBE, SB), and mechanical durability against repeated thermal cycling and handling.
• Integrated lid-interrupt safety circuit automatically de-energizes electrodes upon lid opening—compliant with IEC 61010-1 requirements for laboratory electrical equipment safety.
• Ergonomic base tray with recessed dual handles enables stable, slip-resistant transport of gels during pouring, running, and staining procedures.
• Modular electrode assembly features corrosion-resistant stainless-steel wires mounted on detachable side frames—enabling tool-free cleaning, alignment verification, or replacement without compromising chamber integrity.
• Four-level adjustable leveling feet provide precise horizontal plane calibration across uneven benchtops, minimizing buffer meniscus distortion and band skewing.
• Pre-aligned, snap-fit gel caster eliminates silicone grease application or tape sealing; accommodates both 1.0 mm and 1.8 mm gel thicknesses with standardized comb compatibility.
• Optimized buffer reservoir geometry ensures uniform current density distribution across the full 245 × 250 mm gel surface, reducing edge effects and improving inter-lane reproducibility.

Sample Compatibility & Compliance

The JY-SPHT supports standard agarose concentrations (0.7–3.0%) and native or denaturing polyacrylamide gels (3–20%). Its 364-well maximum capacity is achieved using comb configurations optimized for multi-sample workflows—including 19-, 26-, 44-, and 52-well patterns—each validated for consistent well depth and spacing per ISO 13485-aligned manufacturing controls. The system is compatible with ethidium bromide, SYBR Safe, GelRed, and other industry-standard nucleic acid stains. While not classified as a medical device, its materials meet USP Class VI biocompatibility criteria, and the polycarbonate housing complies with RoHS Directive 2011/65/EU for hazardous substance restrictions.

Software & Data Management

As a benchtop electrophoresis chamber, the JY-SPHT operates independently of proprietary software or digital interfaces. It is fully compatible with third-party gel documentation systems (e.g., Bio-Rad ChemiDoc, Azure Biosystems cSeries, UVP VisionWorks) via standard UV transilluminator integration. All mechanical adjustments—leveling, comb insertion, and lid engagement—are tactile and repeatable, supporting GLP-compliant manual logbook entries. No firmware, cloud connectivity, or electronic audit trail is implemented, aligning with laboratories maintaining paper-based SOPs under ISO/IEC 17025 or CLIA regulatory frameworks.

Applications

• Routine DNA fragment sizing and purity assessment in academic teaching labs and core facilities.
• High-throughput screening of CRISPR editing outcomes using multiplexed gel lanes.
• Quality control of plasmid preps, PCR amplifications, and RT-PCR products prior to sequencing or cloning.
• Restriction mapping validation and Southern blotting prep workflows.
• Student training in fundamental electrophoretic theory—including mobility vs. log MW relationships and buffer ion migration dynamics.

FAQ

Is the JY-SPHT compatible with power supplies from other manufacturers?

Yes—any DC power supply delivering 0–300 V and 0–500 mA can be used, provided output leads are fitted with standard banana-to-alligator clip adapters.
Can I run two gels simultaneously in the same tank?

No—the chamber is engineered for single-gel operation; dual-gel configurations compromise buffer conductivity homogeneity and violate ASTM D5225-18 guidelines for electrophoretic resolution validation.
Does the system include a UV-transparent lid?

No—UV visualization requires external transillumination; the lid is opaque to prevent operator exposure and maintain darkroom compatibility.
What maintenance is required for long-term reliability?

Rinse electrodes and chamber interior with deionized water after each use; inspect comb teeth for polymer residue quarterly; store inverted to prevent dust accumulation in buffer channels.
Is CE or FDA 510(k) certification available?

The JY-SPHT is not CE-marked or FDA-listed, as it falls outside the scope of IVDR or 21 CFR Part 866. It is supplied for research use only (RUO), consistent with global life science instrument classification standards.