Junyi JY-ZY7 Semi-Dry Transfer Electrophoresis Tank

Overview

The Junyi JY-ZY7 Semi-Dry Transfer Electrophoresis Tank is an engineered platform designed for efficient and reproducible electrophoretic transfer of proteins and nucleic acids from polyacrylamide or agarose gels onto solid supports—including nitrocellulose, PVDF, and nylon membranes. Unlike wet transfer systems, the JY-ZY7 employs a semi-dry configuration in which gel-membrane sandwiches are placed directly between two parallel electrode plates immersed in minimal buffer volume, enabling rapid, low-resistance current pathways. This architecture reduces transfer time by up to 70% compared to conventional wet blotting while maintaining high retention of antigenicity and structural integrity of transferred biomolecules. The system operates on standard DC power supplies (not included) and is compatible with both constant-current and constant-voltage protocols. Its compact footprint and modular design make it suitable for routine use in molecular biology laboratories, core facilities, and teaching environments where throughput, safety, and compatibility with existing electrophoresis infrastructure are critical.

Key Features

• Optical-grade polycarbonate housing—single-piece molded for chemical resistance, impact durability, and full visual monitoring of transfer progress;
• Optimized electrode spacing (≤3 mm)—minimizes electrical resistance and Joule heating, enabling faster transfer at lower voltages (typically 15–25 V) without compromising transfer efficiency;
• Color-coded transfer夹板 (blue/red polarity indicators) and electrode terminals—ensures correct orientation of gel-membrane stacks to prevent reversed or failed transfers;
• Lid-integrated safety switch—automatically interrupts power supply upon lid opening, meeting IEC 61010-1 requirements for laboratory electrical equipment safety;
• Tool-free removable electrode plates—facilitates cleaning, inspection, and replacement without disassembly of the main chassis;
• Modular interoperability—mechanically and dimensionally compatible with Junyi JY-JX5 and JY-CZ-B series electrophoresis buffer tanks and lids, allowing shared buffer reservoirs and standardized workflows.

Sample Compatibility & Compliance

The JY-ZY7 accommodates standard mini-gel formats up to 216 mm (W) × 110 mm (L), supporting common pre-cast and hand-cast SDS-PAGE, native PAGE, and agarose gels. It is validated for transfer of proteins ranging from 10 kDa to >250 kDa, including histones, membrane proteins, and phosphorylated isoforms, when used with appropriate transfer buffers (e.g., CAPS, Tris-Glycine-Methanol, or low-pH acetate-based systems). The unit complies with general laboratory safety standards under GB/T 29814–2013 (Chinese national standard for electrophoresis apparatus) and conforms to electromagnetic compatibility (EMC) Class B per GB 4824–2019. While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11, its mechanical and electrical design supports GLP-aligned documentation practices when integrated into validated laboratory workflows.

Software & Data Management

The JY-ZY7 is a hardware-only transfer platform and does not incorporate embedded firmware, touchscreen interface, or digital connectivity. All operational parameters—including voltage, current, duration, and buffer composition—are set externally via compatible DC power supplies (e.g., Junyi JY-300E, Bio-Rad PowerPac Basic, or Thermo Fisher Owl E200). Users maintain full experimental traceability through manual logbooks or LIMS-integrated electronic records. For regulatory environments requiring audit trails, integration with external programmable power supplies featuring timestamped output logging and password-protected protocol storage is recommended.

Applications

• Western blotting—high-efficiency transfer of denatured and native proteins for subsequent antibody probing;
• Eastern blotting—transfer of post-translationally modified proteins for lectin or chemical detection;
• Southern/Northern blotting—DNA/RNA immobilization prior to hybridization with labeled probes;
• Preparative electroblotting—recovery of resolved proteins from gels for mass spectrometry or functional reconstitution;
• Teaching laboratories—robust, intuitive design supports student training in fundamental molecular transfer techniques under supervised conditions.

FAQ

Is the JY-ZY7 compatible with power supplies from other manufacturers?
Yes—it accepts standard DC outputs (0–30 V, 0–1.5 A) via banana jack inputs and is routinely used with power supplies from Bio-Rad, C.B.S. Scientific, and Owl Separations.
Can it be used for wet transfer?
No—the JY-ZY7 is strictly a semi-dry transfer system; it lacks a buffer tank cavity and cooling jacket required for submerged wet transfer protocols.
What maintenance is required for the electrodes?
Electrodes should be rinsed with deionized water after each use and inspected for pitting or oxidation; replacement plates are available as service parts (P/N: JY-ZY7-EP-KIT).
Does it support temperature monitoring during transfer?
No—temperature sensing is not integrated; users should monitor ambient lab temperature and limit run times to prevent excessive heat buildup in high-conductivity buffers.
Is the tank autoclavable?
No—polycarbonate components are not autoclave-rated; cleaning is performed with 70% ethanol or mild detergent followed by thorough rinsing with DI water.