Junyi JY300 Electrophoresis Power Supply

Overview

The Junyi JY300 Electrophoresis Power Supply is a compact, microprocessor-controlled DC power source engineered for reliable and precise operation in routine nucleic acid and protein electrophoresis applications. It operates on the fundamental principle of applying a controlled electric field across a gel matrix—such as agarose or polyacrylamide—to drive charged biomolecules toward oppositely charged electrodes at rates determined by molecular size, conformation, and charge-to-mass ratio. Designed specifically for horizontal slab gels, mini vertical electrophoresis systems, and acetate membrane electrophoresis, the JY300 delivers stable, low-noise DC output without ripple interference that could compromise band resolution or cause gel overheating. Its dual-mode architecture supports both constant voltage (CV) and constant current (CC) operation, enabling users to select the optimal mode based on experimental requirements: CV for consistent migration speed under varying gel resistance, and CC for uniform current density when running multiple gels in parallel or during extended runs where buffer conductivity changes over time.

Key Features

• Intelligent microprocessor control with touch-sensitive keypad interface and one-piece molded housing for enhanced durability and electromagnetic interference (EMI) shielding
• Simultaneous dual LED digital display showing real-time voltage and current values, eliminating ambiguity during method setup and monitoring
• Programmable timer with precision range from 1 minute to 9 hours and 59 minutes, supporting unattended overnight runs with automatic shutdown
• Auto-ranging logic: when constant voltage mode is selected, the unit dynamically adjusts output current up to its maximum limit (300 mA); conversely, in constant current mode, voltage is automatically elevated to maintain set current within system limits (up to 300 V)
• Comprehensive safety architecture including overload protection, open-circuit (no-load) detection, and ground-fault leakage current cutoff—critical for lab personnel safety and equipment longevity
• Memory retention function preserves last-used settings (voltage/current/timer) across power cycles, reducing setup time between experiments
• Two independent output port pairs support simultaneous operation of two electrophoresis units or redundant connections for improved reliability

Sample Compatibility & Compliance

The JY300 is compatible with standard electrophoresis chambers used in academic teaching labs, quality control environments, and early-stage R&D workflows—including but not limited to mini vertical SDS-PAGE tanks, horizontal agarose gel trays (up to 15 × 15 cm), and cellulose acetate membrane systems. While it does not integrate with imaging or UV transilluminators, its clean, regulated DC output ensures reproducible migration patterns essential for qualitative and semi-quantitative analysis. The instrument complies with GB/T 20945–2013 (Chinese national standard for electrical safety of laboratory equipment) and meets IEC 61010-1:2010 requirements for measurement, control, and laboratory use. Though not certified for GMP or GLP-regulated production environments, its stable performance and audit-ready parameter logging (via external data loggers) make it suitable for ISO/IEC 17025-accredited testing laboratories performing routine biomolecular separation.

Software & Data Management

The JY300 operates as a standalone hardware platform with no embedded software or network connectivity. All operational parameters are configured manually via front-panel controls and displayed locally. For laboratories requiring electronic recordkeeping, the unit’s dual digital readout enables manual transcription into LIMS or ELN systems. When paired with compatible data acquisition modules (e.g., USB-connected multimeters or analog-to-digital converters), real-time voltage and current traces can be logged for method validation, troubleshooting, or regulatory documentation. No proprietary drivers or cloud services are required; raw numeric outputs conform to standard SI units and require no conversion for inclusion in analytical reports compliant with FDA 21 CFR Part 11 Annex 11 expectations for traceability.

Applications

• Routine agarose gel electrophoresis for DNA fragment sizing (0.1–20 kb), PCR product verification, and plasmid analysis
• Mini vertical SDS-PAGE for protein molecular weight determination, Western blot sample preparation, and Coomassie/silver staining workflows
• Cellulose acetate electrophoresis for serum protein fractionation (albumin, α1-globulin, α2-globulin, β-globulin, γ-globulin) in clinical biochemistry teaching and screening assays
• Isotachophoresis and basic native PAGE setups where stable current delivery is critical for sharp zone formation
• Electrophoretic mobility shift assays (EMSA) requiring precise, low-drift voltage application over extended durations

FAQ

What electrophoresis chamber types are compatible with the JY300?
The JY300 supports standard mini vertical electrophoresis tanks (e.g., Bio-Rad Mini-PROTEAN–compatible), horizontal agarose gel trays up to 15 × 15 cm, and cellulose acetate membrane holders. It is not rated for high-voltage sequencing or large-format vertical systems.
Does the JY300 support programmable ramping or gradient protocols?
No—the JY300 provides only fixed-setpoint constant voltage or constant current output. It does not offer time-programmed voltage/current ramps or multi-step protocols.
Can the JY300 be used for electroblotting or electroelution?
Yes, within its 90 W power envelope and thermal design limits; however, extended high-current transfer runs (>1 h at >200 mA) may trigger thermal protection depending on ambient conditions and cooling efficiency of the transfer apparatus.
Is there a calibration certificate available for the JY300?
Factory calibration is performed per GB/T 20945–2013 prior to shipment. A traceable calibration report (NIST-traceable standards) can be requested at time of order for an additional fee.
What maintenance is required for long-term reliability?
Routine cleaning of output terminals and ventilation grilles is recommended. No internal user-serviceable components exist; periodic verification of output accuracy using a calibrated digital multimeter is advised every 12 months in regulated environments.