Junyi JY5000E High-Voltage Electrophoresis Power Supply

Overview

The Junyi JY5000E High-Voltage Electrophoresis Power Supply is an engineered platform for precise, reproducible delivery of electrical energy in nucleic acid and protein separation workflows. Designed for compatibility with vertical slab gels, sequencing gels (including SSR and AFLP applications), two-dimensional electrophoresis (2-DE), and isoelectric focusing (IEF), the instrument operates on fundamental principles of controlled electric field generation across polyacrylamide or agarose matrices. Its triple-mode output architecture—supporting constant voltage, constant current, and constant power modes—enables method-specific optimization while maintaining strict adherence to Ohm’s law relationships under dynamic load conditions. The unit delivers up to 5000 V at 200 mA, making it suitable for high-resolution denaturing gradient gels, long-run native PAGE, and capillary electrophoresis support systems where stable, low-drift voltage profiles are essential.

Key Features

• Dual-core microprocessor control ensures real-time regulation of output parameters with <1% deviation under varying gel resistance conditions.
• Intuitive touch-key interface with backlit LCD provides simultaneous display of setpoint and actual values—critical for GLP-compliant documentation and troubleshooting.
• 100 programmable method storage supports laboratory workflow standardization and reduces operator-dependent variability across shifts or users.
• Stepwise electrophoresis programming allows multi-stage runs (e.g., initial low-voltage equilibration followed by high-voltage separation), with up to 10 sequential steps and 10 sub-programs per step.
• Real-time pause-and-adjust functionality preserves method integrity during interruptions; fine-tuning during pause mode does not compromise output stability upon resumption.
• Integrated safety architecture includes automatic shutdown on open-circuit detection, overcurrent limiting, ground-fault leakage protection, and thermal cut-off—meeting IEC 61010-1 requirements for laboratory electrical equipment.
• Optional gel temperature sensor interface enables correlation of run performance with thermal drift—a key parameter in reproducibility assessment for IEF and high-voltage SDS-PAGE.

Sample Compatibility & Compliance

The JY5000E is validated for use with commercially available vertical electrophoresis tanks (e.g., Bio-Rad Mini-PROTEAN, Thermo Fisher Owl E1, C.B.S. Scientific models) and custom-built units conforming to standard electrode spacing and buffer chamber volumes. It complies with ISO/IEC 17025 general requirements for competence of testing and calibration laboratories when used within documented SOPs. While not FDA 21 CFR Part 11–certified out-of-the-box, its audit-trail-capable method storage, timestamped protocol execution, and non-volatile memory align with GxP data integrity expectations when paired with appropriate lab information management systems (LIMS). All electrical safety certifications follow GB 4793.1–2019 (equivalent to IEC 61010-1:2010).

Software & Data Management

The JY5000E operates as a standalone instrument with embedded firmware—no PC dependency required for routine operation. All 100 stored methods are retained in non-volatile memory after power cycling. Each method entry logs creation date, last modification time, and cumulative runtime statistics (V·hr totals). Export of method parameters is possible via manual transcription from the LCD interface; optional RS-232 or USB-to-serial adapters (sold separately) enable integration with third-party acquisition software for automated logging into electronic lab notebooks (ELN) or LIMS platforms. Firmware updates are performed via secure UART bootloader interface using manufacturer-provided utilities.

Applications

• High-resolution DNA fragment analysis in SSR and AFLP genotyping workflows requiring extended high-voltage runs (e.g., 3000–5000 V for 4–6 h).
• Two-dimensional electrophoresis: first-dimension IEF strips (pH 3–10) followed by second-dimension SDS-PAGE under controlled power ramping.
• Preparative native PAGE for intact protein complex isolation, where constant power mode prevents localized overheating in thick gels.
• Capillary electrophoresis system auxiliary power supply for UV-transparent fused silica capillaries operating above 30 kV/cm field strength.
• Electroblotting optimization studies requiring precise volt-hour dosing for quantitative transfer efficiency modeling.

FAQ

Does the JY5000E support remote control or computer interfacing?
Yes—via optional RS-232 or USB-to-serial adapter; no proprietary software is bundled, but ASCII command protocol documentation is provided for custom integration.
Is gel temperature monitoring included by default?
No—the temperature sensor is an optional accessory; the instrument provides a calibrated analog input port compatible with industry-standard 10 kΩ NTC thermistors.
Can the JY5000E be used with horizontal agarose gel systems?
It is technically capable, but not recommended for routine use due to insufficient current capacity for large-format (>20 × 20 cm) gels; best suited for vertical and mini-gel configurations.
What safety standards does the JY5000E meet?
It conforms to GB 4793.1–2019 (equivalent to IEC 61010-1:2010) for electrical safety in laboratory equipment, including dielectric strength testing, creepage/clearance verification, and protective earth continuity checks.
How is method validation supported for regulated environments?
Method storage includes immutable timestamps and cumulative V·hr tracking; users must implement procedural controls (e.g., dual-operator verification, printed log sheets) to satisfy ALCOA+ data integrity principles in GMP/GLP settings.