Keebio Keebio-96S Polymerase Chain Reaction Instrument
| Brand | Keebio |
|---|---|
| Origin | Shanghai, China |
| Model | Keebio-96S |
| Instrument Type | Standard Thermal Cycler |
| Sample Capacity | 96-well (0.2 mL format) |
| Max Heating/Cooling Rate | 5 °C/s |
| Temperature Accuracy | ≤ ±0.1 °C at 55 °C |
| Block Uniformity | ≤ ±0.2 °C at 55 °C |
| Gradient Range | 1–30 °C (±0.3 °C accuracy), 30–105 °C |
| Temperature Control Modes | Block & Tube |
| Programmable Ramp Rate | 0.1–5 °C/s |
| Max Cycles | 100 |
| Time Increment/Decrement | 1–600 s |
| Temp Increment/Decrement | 0.1–10 °C |
| Program Storage | 20,000 protocols |
| 4 °C Hold Function | Yes |
| Power Input | 100–240 VAC, 50/60 Hz |
| Output Power | 700 W |
| Dimensions (D×W×H) | 293 × 190 × 183 mm |
| Net Weight | 5.5 kg |
Overview
The Keebio Keebio-96S Polymerase Chain Reaction Instrument is a benchtop thermal cycler engineered for precision, reliability, and operational efficiency in molecular biology laboratories. It employs Peltier-based thermoelectric heating and cooling technology to deliver rapid, accurate, and reproducible temperature transitions across the full PCR profile range (0–105 °C). Designed for routine amplification workflows—including standard PCR, touchdown PCR, long-range PCR, and nested PCR—the instrument supports rigorous experimental requirements while maintaining compliance with fundamental laboratory quality practices. Its compact footprint, metal chassis construction, and forward-to-rear airflow architecture ensure stable thermal performance and extended operational durability under continuous use.
Key Features
- 7-inch fully laminated capacitive touchscreen with intuitive UI—enables drag-and-zoom navigation, multi-step program editing, and real-time thermal curve preview within a single interface
- Adaptive heated lid (30–115 °C, ±0.1 °C display resolution) automatically adjusts pressure and temperature to accommodate diverse consumables including 0.2 mL tubes, 8-tube strips, and full-skirt 96-well PCR plates
- Optimized aluminum heating block with proprietary thermal mass distribution—achieves ≤ ±0.2 °C uniformity across all 96 positions at 55 °C without auxiliary preheating
- Dual temperature control modes (Block and Tube) allow user-selectable referencing strategies for improved assay consistency
- Programmable ramp rates from 0.1 to 5 °C/s support fine-tuned optimization of denaturation, annealing, and extension phases
- Comprehensive data integrity safeguards: password-protected user accounts, automatic power-fail recovery, persistent 4 °C hold function, and post-run auto-lid closure
- Onboard storage for up to 20,000 user-defined protocols with timestamped versioning and editable metadata fields
Sample Compatibility & Compliance
The Keebio-96S accommodates standard 96-well PCR formats (0.2 mL volume), individual 0.2 mL tubes, and 8-tube strips—excluding full-skirt plates due to mechanical clearance constraints. Its thermal calibration protocol aligns with ISO/IEC 17025 traceability principles for temperature-controlled laboratory equipment. While not certified to FDA 21 CFR Part 11 out-of-the-box, the system supports audit-ready operation when integrated into GLP/GMP environments via external LIMS or ELN systems. All firmware updates are digitally signed and logged; temperature validation reports can be exported in CSV format for internal QA review.
Software & Data Management
The instrument operates independently without PC dependency, yet supports USB-based protocol import/export for cross-platform workflow synchronization. Each saved program includes embedded thermal profile visualization, cycle-by-cycle step annotation, and configurable pause points. User access levels are enforced through encrypted password protection—ensuring separation of administrative, operator, and trainee permissions. All runtime events—including lid status, temperature deviations > ±0.3 °C, and power interruptions—are timestamped and retained in non-volatile memory for up to 30 days. Exported data files include instrument ID, operator ID, date/time stamps, and raw thermal logs compatible with third-party analysis tools.
Applications
The Keebio-96S serves core applications across academic research, clinical diagnostics, public health testing, and biotechnology education. It is routinely deployed for DNA template amplification prior to Sanger sequencing, qPCR assay development, genotyping assays (e.g., SNP detection), pathogen screening (including viral load quantification), and forensic STR profiling. Its gradient capability (1–30 °C span, ±0.3 °C accuracy) facilitates robust annealing temperature optimization for primer pairs with variable Tm. The instrument’s low thermal inertia and high positional uniformity make it suitable for high-throughput teaching labs requiring consistent inter-user reproducibility across student workstations.
FAQ
Does the Keebio-96S support gradient PCR?
Yes—it offers programmable gradient spans from 1 to 30 °C across the block, with temperature accuracy of ±0.3 °C at any setpoint between 30 and 105 °C.
What consumables are compatible with the 96-well block?
0.2 mL single tubes, 8-tube strips, and semi-skirted or unskirted 96-well PCR plates. Full-skirted plates are mechanically incompatible.
Is remote monitoring or network connectivity available?
No native Ethernet or Wi-Fi interface is included; however, USB-host functionality enables integration with lab automation software via custom scripting.
How is temperature calibration verified?
Calibration follows manufacturer-specified procedures using NIST-traceable reference probes; users may perform periodic verification using external calibrated thermistors inserted into designated wells.
Can the instrument be used for long-range PCR (>10 kb)?
Yes—the combination of precise ramp-rate control (down to 0.1 °C/s), tight block uniformity, and stable 4 °C hold post-run supports extended extension times and optimized cycling conditions required for long-amplicon synthesis.
