PerkinElmer Flexar UHPLC System

Overview

The PerkinElmer Flexar UHPLC System is a high-performance, modular liquid chromatography platform engineered for precision, reproducibility, and regulatory compliance in analytical laboratories. Built upon PerkinElmer’s proven Flexar architecture—originally introduced in 2013—the system employs low-dispersion fluidic design, high-pressure gradient capability (up to 1,000 bar), and real-time pressure monitoring to support both conventional HPLC and ultra-high-performance liquid chromatography (UHPLC) applications. Its core measurement principle relies on differential UV/Vis absorbance detection across a wavelength range of 190–900 nm, enabling quantitative and qualitative analysis of small molecules, pharmaceuticals, natural products, and environmental analytes. Designed and manufactured in Canada, the Flexar platform adheres to international design standards aligned with ISO 9001-certified production processes and meets mechanical and electrical safety requirements per IEC 61010-1.

Key Features

• Modular architecture supporting flexible configuration: solvent manager, autosampler, column oven, binary or quaternary pump, and UV/Vis detector can be independently validated and replaced.
• Quaternary solvent manager (P/N N2600580) with integrated nitrogen-based degassing, delivering stable baseline performance and minimizing bubble-induced retention time drift.
• Autosampler (P/N N2930660) with 100-position vial capacity, programmable injection volume (0.1–100 µL), and needle wash station—designed for high-throughput routine QC and method development workflows.
• Column oven (P/N N2601680) offering precise temperature control from ambient +5 °C to 85 °C (±0.2 °C stability), critical for method robustness and retention time reproducibility.
• Binary LC pump (P/N N2910401) capable of flow rates from 0.001 to 10.0 mL/min with pulse dampening and active backpressure regulation for consistent gradient delivery.
• UV/Vis detector featuring dual-beam optics, variable pathlength cell (10 mm standard), and data acquisition at up to 100 Hz—optimized for peak fidelity and signal-to-noise ratio in complex matrices.

Sample Compatibility & Compliance

The Flexar UHPLC system accommodates standard 2.1–4.6 mm ID analytical columns packed with C18, phenyl-hexyl, HILIC, or ion-exchange phases. It supports mobile phases including aqueous buffers (e.g., ammonium formate, phosphate), organic modifiers (acetonitrile, methanol), and additives compatible with stainless-steel and PEEK-wetted components. All modules are compliant with GLP/GMP operational prerequisites and support audit-trail-enabled software configurations meeting FDA 21 CFR Part 11 requirements when deployed with PerkinElmer’s Turbochrom CDS or third-party validated chromatography data systems. Instrument qualification documentation—including Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ) templates—is available upon request to facilitate laboratory validation under ISO/IEC 17025 or USP guidelines.

Software & Data Management

The system operates natively with PerkinElmer Turbochrom CDS (v7.4 or later), providing full method development, sequence control, peak integration, calibration curve generation, and electronic signature functionality. Raw data files (.raw) are stored in vendor-neutral formats compatible with third-party processing tools (e.g., Chromeleon, OpenLab CDS). All instrument modules log timestamped event records—including pressure spikes, leak alerts, temperature deviations, and valve actuations—enabling traceability for root-cause analysis. Data integrity safeguards include encrypted storage, user role-based access control (admin/operator/auditor profiles), and immutable audit trails synchronized across hardware modules.

Applications

This refurbished Flexar UHPLC system is routinely deployed in pharmaceutical quality control labs for assay validation, dissolution testing, and impurity profiling per ICH Q2(R2) and USP monographs. It serves as a reliable platform for food safety testing (e.g., pesticide residue screening per AOAC 2007.01), environmental analysis (PAHs, PCBs per EPA Method 8330B), and academic research requiring high-resolution separation of polar metabolites or synthetic intermediates. Its modularity allows seamless integration into hybrid workflows—for example, coupling with mass spectrometry via post-column flow splitting or direct ESI interface adaptation.

FAQ

Is this system fully tested and certified prior to shipment?
Yes—each Flexar UHPLC system undergoes comprehensive functional verification at SpectraLab Scientific’s ISO 17025-accredited facility, including pressure integrity checks, gradient accuracy assessment, detector linearity validation (using caffeine standards), and autosampler precision testing (RSD ≤ 0.5% at 10 µL).

What documentation accompanies the instrument?
Customers receive a Certificate of Inspection, detailed test report, module-specific service logs, and a 6-month limited warranty covering parts and labor for defects in materials or workmanship.

Can the system be configured for GxP-regulated environments?
Yes—when paired with Turbochrom CDS v7.4+ and configured with appropriate user access controls and audit trail settings, the system satisfies key elements of 21 CFR Part 11 and EU Annex 11 for electronic records and signatures.

Are spare parts and consumables available?
All major consumables—including seal kits, check valves, purge filters, and column frits—are stocked and available under PerkinElmer OEM part numbers; extended support contracts can be arranged upon request.