Raykol Auto Prep 200 Fully Automated Liquid Sample Preparation Workstation

Overview

The Raykol Auto Prep 200 Fully Automated Liquid Sample Preparation Workstation is an engineered solution for high-integrity, reproducible liquid handling in regulated and research-intensive laboratory environments. Built on a dual-syringe pump architecture with gravimetric and volumetric calibration traceability, the system performs precise liquid dispensing, serial dilution, standard curve generation, spike addition, reagent aliquoting, and multi-step stock solution preparation — all under programmable, closed-system conditions. Its design adheres to core principles of analytical sample integrity: minimizing operator-induced variability, suppressing solvent volatility effects, preventing carryover contamination, and ensuring full auditability of preparation parameters. The workstation operates without manual intervention during method execution, supporting unattended overnight runs while maintaining temperature-stabilized (semiconductor-cooled) and optional light-shielded environments — critical for light- or heat-sensitive analytes such as pesticides, pharmaceutical impurities, or unstable metabolites.

Key Features

• Dual high-precision syringe pump system with auto-selection of optimal displacement range (10 µL–25 mL), enabling consistent accuracy of ±1.0% across the full dynamic range
• Integrated semiconductor-based cooling module maintains sample deck temperature between 4 °C and ambient, mitigating evaporation losses during prolonged preparation cycles
• Puncture-style needle interface allows direct-through-cap sampling from sealed vials — eliminating headspace exposure and preserving volatile compound integrity
• Multi-stage needle washing protocol (up to 5 rinse steps with configurable solvents), emulating HPLC autosampler-level decontamination rigor to reduce carryover to <0.001%
• Disposable tip-based pipetting mode with automated tip loading, ejection, and waste disposal — fully eliminating cross-sample contamination risk
• Real-time volumetric feedback display for both pumps, synchronized with method log timestamps and instrument status indicators
• Light-tight enclosure option available for photolabile compounds (e.g., retinoids, nitroaromatics, certain APIs), compliant with ICH Q5C stability testing guidelines

Sample Compatibility & Compliance

The Auto Prep 200 accommodates standard 12 × 75 mm, 15 mL, and 50 mL conical tubes, 96-well and 384-well microplates, and custom vial formats via configurable deck adapters. It supports aqueous buffers, organic solvents (including acetonitrile, methanol, and ethyl acetate), viscous solutions up to 50 cP, and mildly corrosive reagents (pH 2–12). All liquid contact materials are chemically inert (PTFE, PEEK, borosilicate glass, and stainless steel 316). The system meets mechanical safety requirements per IEC 61010-1 and electromagnetic compatibility per EN 61326-1. Data integrity features include user-level access control, electronic signatures, and immutable audit trails — aligning with FDA 21 CFR Part 11, EU Annex 11, and GLP/GMP documentation expectations for method validation and routine QC use.

Software & Data Management

The Raykol PrepControl™ software provides a validated, wizard-driven interface for method development — requiring only initial stock concentration, target concentration, and final volume inputs. The software automatically calculates intermediate dilutions, optimizes pipetting sequences, and generates step-by-step execution logs. All methods are stored with version control, time/date stamps, and operator ID metadata. Post-run reports include quantitative summary tables, pump displacement verification data, temperature history graphs, and pass/fail flags against pre-set acceptance criteria (e.g., %RSD ≤ 2.0 for replicate standards). Raw data exports comply with ASTM E1998 and ISO/IEC 17025 reporting structures, supporting integration into LIMS and ELN platforms via CSV or XML schema.

Applications

• Preparation of certified reference material (CRM) dilutions for GC-MS, LC-MS/MS, and ICP-MS calibration
• Automated generation of multi-point calibration curves (5–12 points) with internal standard spiking
• High-throughput sample spiking for recovery studies and matrix-matched calibration
• Stability-indicating dilution series for forced degradation product quantification
• Batched preparation of QC check standards for environmental residue analysis (e.g., EPA Method 525.3, 8270)
• Standardized dilution workflows in clinical toxicology labs performing therapeutic drug monitoring (TDM)

FAQ

Does the Auto Prep 200 support method validation per ICH Q2(R2)?
Yes — the system enables full validation of accuracy, precision, linearity, and robustness through built-in replicate preparation modes, statistical reporting, and raw data traceability.
Can it handle viscous samples such as glycerol or polymer solutions?
It supports liquids up to 50 cP using low-speed aspiration/dispense profiles; viscosity compensation parameters are configurable in method settings.
Is remote monitoring supported for unattended operation?
Yes — real-time status dashboard, email/SMS alerts for completion or error events, and secure web-based access via TLS 1.2 are included.
What regulatory documentation is provided for installation qualification (IQ) and operational qualification (OQ)?
Raykol supplies IQ/OQ protocols, factory calibration certificates (NIST-traceable syringe verification), and software validation summaries compliant with GAMP 5.
How is carryover verified during cleaning sequence validation?
Carryover testing follows USP guidance using high-concentration challenge solutions; residual detection limits are confirmed via post-wash blank injections on connected analytical instruments.