Zhichu ZCLY-100 Vertical Stackable CO₂ Orbital Shaker Incubator
| Brand | Zhichu |
|---|---|
| Origin | Guangdong, China |
| Manufacturer Type | Authorized Distributor |
| Instrument Category | Domestic |
| Model | ZCLY-100 |
| Pricing | Upon Request |
| Instrument Type | CO₂-Controlled Constant-Temperature Orbital Shaker |
| Timer Range | 0–999.9 h |
| Oscillation Frequency | 10–300 rpm |
| Frequency Accuracy | ±1 rpm |
| Orbit Diameter | Ø50 mm (standard), Ø26 mm (optional) |
| Temperature Range | 4–60 °C (ambient 23–25 °C) |
| Temperature Uniformity | ±0.5 °C (at 37 °C) |
| Temperature Resolution | ±0.1 °C |
| CO₂ Control Range | 0–20% |
| CO₂ Control Accuracy | ±0.1% |
| Chamber Interior | Seamless 304 Stainless Steel with R-corner Design |
| Shelves | 3 Adjustable Platforms |
| Max Capacity (per shelf) | 250 mL × 20 / 500 mL × 12 / 1000 mL × 8 / 2 L × 5 / 5 L × 2 (stainless steel fixtures) |
| Display | LCD Touchscreen Interface |
| Data Logging | 1-min Interval, Up to 1 Year Storage |
| Connectivity | USB Export Port |
| Power Supply | AC 220 V ±10%, 50–60 Hz |
| Dimensions (W×D×H) | 600 × 766 × 998 mm |
| Net Weight | ~125 kg |
| Noise Level | <55 dB(A) |
Overview
The Zhichu ZCLY-100 Vertical Stackable CO₂ Orbital Shaker Incubator integrates precise temperature regulation, controlled CO₂ atmosphere, and uniform orbital shaking into a single compact platform engineered for mammalian cell culture, microbial fermentation, and suspension-based bioprocess development. It operates on the principle of forced-air convection combined with infrared CO₂ sensing and PID-driven thermal feedback, ensuring stable environmental conditions across all three independently adjustable shelves. Unlike conventional shakers, the ZCLY-100 employs a triple-offset cam drive system that delivers smooth, low-vibration motion at frequencies from 10 to 300 rpm—critical for maintaining cell viability during long-term incubation. Its vertical stackable architecture supports dual-unit configuration without requiring additional floor space, making it suitable for high-density lab environments where footprint efficiency is constrained.
Key Features
- Triple-offset cam drive mechanism ensures mechanical stability, minimal harmonic resonance, and extended service life under continuous operation.
- Integrated infrared CO₂ sensor (imported) enables real-time monitoring and closed-loop concentration control within ±0.1% accuracy across 0–20% range; optional photobioreactor module supports light intensity modulation (0–10,000 lux).
- Seamless 304 stainless-steel chamber with R-corner geometry eliminates bacterial harborage points and permits full-volume rinsing—validated for ISO 14644-1 Class 5 cleanroom-compatible cleaning protocols.
- PID-controlled refrigeration system using R134a-free eco-friendly refrigerant and high-efficiency compressor maintains temperature setpoints from 4 °C to 60 °C with ±0.1 °C resolution and ±0.5 °C uniformity at 37 °C.
- LCD touchscreen interface displays real-time values for temperature, CO₂ concentration, speed, elapsed time, and remaining timer—all on one screen without navigation toggling; includes operator lockout via password encryption.
- Comprehensive data logging records temperature, speed, and CO₂ levels every 60 seconds for up to 12 months; exportable via USB to CSV or Excel-compatible formats for audit-ready traceability.
- Dual heating zones (glass and door) prevent condensation during low-temperature/high-humidity operation; UV-C sterilization cycle (timed, 15–30 min) is programmable and validated per ISO 15858 for surface microbial reduction ≥99%.
Sample Compatibility & Compliance
The ZCLY-100 accommodates standard Erlenmeyer flasks (250–5000 mL), roller bottles, and multi-well plates across three independently configurable shelves. Each shelf measures 485 × 410 mm and accepts universal plastic or stainless-steel fixtures—enabling rapid reconfiguration between batch cultures and high-throughput screening workflows. The chamber’s seamless construction and autoclavable components comply with GLP documentation requirements and support routine validation per ASTM E2500-13 (Good Manufacturing Practice for Laboratory Equipment). Optional humidity control module extends operational envelope for sensitive primary cell lines requiring >85% RH. All electrical subsystems meet IEC 61010-1 safety standards; CO₂ sensor calibration certificates are traceable to NIST standards upon request.
Software & Data Management
No proprietary software installation is required—the ZCLY-100 operates as a standalone instrument with embedded firmware supporting FDA 21 CFR Part 11-compliant audit trails when connected to networked laboratory information management systems (LIMS). Timestamped event logs capture power interruptions, parameter changes, door openings, alarm triggers (e.g., overtemperature, CO₂ deviation >0.3%), and sterilization cycles. Data export via USB adheres to ISO/IEC 17025 Annex A.3 guidelines for raw data integrity. Remote monitoring capability (via optional Wi-Fi-enabled module) provides TLS-encrypted access through secure web portal—supporting role-based user permissions and session timeout policies aligned with institutional IT security policies.
Applications
- Mammalian cell expansion in serum-free or chemically defined media under physiological CO₂ (5%) and temperature (37 °C) conditions.
- Microbial growth kinetics studies requiring simultaneous pH-independent CO₂ buffering and agitation-induced oxygen transfer enhancement.
- Protein expression optimization in E. coli or yeast strains under controlled aeration and thermal stress regimes.
- Biopharmaceutical process development including seed train scaling, clone selection, and media formulation testing.
- Environmental microbiology assays involving aerobic heterotroph enumeration under standardized incubation parameters per ISO 6222 and APHA Standard Methods 9215B.
FAQ
Can the ZCLY-100 be validated for GMP-compliant production environments?
Yes—its PID-controlled thermal and gas subsystems, electronic audit trail, and stainless-steel chamber support IQ/OQ/PQ execution per Annex 15 of the EU GMP Guide. Calibration templates and URS alignment documentation are available upon order.
Is the CO₂ sensor factory-calibrated and field-serviceable?
The infrared sensor is pre-calibrated against certified gas standards and includes onboard zero/span verification routines. Field recalibration kits and NIST-traceable reference gases are supplied separately.
What is the maximum allowable load per shelf at 300 rpm?
At full speed, maximum recommended mass per shelf is 15 kg—including vessel weight, medium volume, and fixture mass—to preserve mechanical longevity and thermal uniformity.
Does the unit support external CO₂ supply integration?
Yes—dual-stage pressure-regulated inlet port accepts medical-grade CO₂ cylinders or centralized lab gas manifolds; integrated solenoid valve enables automatic cutoff during door opening or fault conditions.
How is temperature uniformity verified across all three shelves?
Factory verification uses 9-point thermocouple mapping per ISO 17025-accredited protocol; users may perform periodic checks using calibrated PT100 probes placed at designated grid positions outlined in the Operation Qualification manual.
