Revvity Cellaca PLX Imaging Cytometry System
| Brand | Revvity |
|---|---|
| Origin | USA |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Origin Category | Imported |
| Model | Cellaca PLX |
| Pricing | Available Upon Request |
Overview
The Revvity Cellaca PLX Imaging Cytometry System is a high-throughput, benchtop imaging cytometer engineered for quantitative single-cell analysis in suspension. Unlike conventional flow cytometers that rely on hydrodynamic focusing and photomultiplier tube (PMT)-based fluorescence detection, the Cellaca PLX employs wide-field fluorescence microscopy coupled with high-resolution CMOS imaging and deterministic cell positioning—enabling label-free brightfield imaging alongside simultaneous six-channel fluorescence quantification. This architecture eliminates sheath fluid requirements and reduces optical path variability, resulting in superior reproducibility for low-volume samples and enhanced sensitivity in detecting rare subpopulations. Designed specifically for translational research and QC/QA applications in biopharma, immuno-oncology, and cell therapy development, the system delivers rapid, standardized, and regulatory-compliant cell characterization without sacrificing morphological context.
Key Features
- Ultra-low sample consumption: Analyzes 15–50 µL of cell suspension per run—reducing sample volume requirements by 10–30× compared to conventional flow cytometry.
- Integrated dual-modality imaging: Simultaneous acquisition of brightfield and six-color fluorescence (405/488/561/640 nm excitation; configurable emission filters) within ≤60 seconds per sample.
- FDA 21 CFR Part 11–compliant software architecture: Supports electronic signatures, role-based user permissions, audit trails, and secure data encryption for GLP/GMP-aligned workflows.
- No routine optical calibration required: Factory-validated illumination uniformity and detector linearity ensure long-term measurement stability with minimal operator intervention.
- Automated workflow integration: Compatible with standard robotic liquid handlers (e.g., Hamilton STAR, Tecan Fluent) via RS-232 and Ethernet interfaces; supports LIS/HIS connectivity through HL7 and ASTM E1384 protocols.
- Pre-optimized, validated assay kits: Includes CE-IVD–marked reagents for immunophenotyping (CD3/CD4/CD8/CD19/CD56), apoptosis (Annexin V/PI), viability (AO/PI dual-stain), and fluorescent protein expression (GFP/RFP/mCherry).
Sample Compatibility & Compliance
The Cellaca PLX accommodates mammalian suspension cells—including primary PBMCs, hybridomas, CAR-T cells, and adherent cells after gentle enzymatic or EDTA-based detachment—within a concentration range of 1×10⁴ to 1×10⁷ cells/mL. Its low-background, black-walled microfluidic cartridges minimize autofluorescence and cross-talk between fluorophores, enabling robust multiplexing even with spectrally overlapping dyes. All hardware, firmware, and Matrix software comply with IEC 61010-1 (safety), IEC 61326-1 (EMC), and ISO 13485:2016 (medical device quality management). The system meets analytical validation requirements outlined in USP , ISO 20387, and ASTM E2577 for cell counting and viability assessment.
Software & Data Management
The Matrix Analysis Software provides an intuitive, wizard-driven interface with preloaded SOP templates for common assays. Each protocol includes step-by-step guidance for staining, loading, and acquisition, with real-time QC feedback (e.g., focus score, cell density, debris threshold). Results are presented as interactive dot plots, histograms, heatmaps, and population gating trees—with export options for FCS 3.1, CSV, PDF, and TIFF formats. Data storage utilizes AES-256 encrypted local SSDs with optional network-attached storage (NAS) mirroring. Audit logs record all user actions—including parameter changes, gate adjustments, and report generation—with timestamped, immutable entries compliant with FDA 21 CFR Part 11 Subpart B requirements.
Applications
- Immunophenotyping of T-cell subsets in adoptive cell therapy manufacturing and release testing.
- Longitudinal viability monitoring during bioreactor harvest and cryopreservation recovery.
- Apoptosis kinetics profiling in compound screening assays using Annexin V/7-AAD dual staining.
- Fluorescent reporter validation in CRISPR-edited cell lines and iPSC-derived models.
- Process consistency verification across upstream bioprocessing steps—from inoculum expansion to final formulation.
FAQ
Does the Cellaca PLX require daily calibration or alignment procedures?
No. The system undergoes factory calibration for illumination intensity, camera gain, and spectral crosstalk compensation. Users perform only quarterly verification using supplied reference beads.
Can third-party antibodies or dyes be used with the Cellaca PLX?
Yes—provided they are compatible with the instrument’s excitation/emission filter set and do not exceed 10⁶ particles/mL concentration to avoid image saturation.
Is remote software update support available?
Yes. Revvity provides secure over-the-air (OTA) updates via authenticated HTTPS connections, with version rollback capability and change-log documentation.
What level of IT infrastructure is required for enterprise deployment?
A minimum of Windows Server 2019 or later with Active Directory integration, TLS 1.2+ encryption, and SQL Server 2017+ for centralized database hosting.
Are training materials available in languages other than English?
Yes. The Matrix software UI supports 28 localized language packs (including Simplified Chinese, Japanese, German, French, and Spanish); technical documentation and video SOPs are available in 12 languages.
