Revvity Chemagic 360 Automated Nucleic Acid Extraction System
| Brand | Revvity |
|---|---|
| Origin | USA |
| Manufacturer | Revvity, Inc. |
| Product Category | Imported Instrument |
| Model | Chemagic 360 |
| Instrument Type | Magnetic Bead-Based Extraction |
| Automation Level | Dedicated Fully Automatic |
| Throughput | 1–96 samples per run |
| Sample Volume Range | 10 µL – 10 mL |
Overview
The Revvity Chemagic 360 Automated Nucleic Acid Extraction System is an engineered platform for high-fidelity, walk-away purification of DNA and RNA from diverse biological matrices—including whole blood, saliva, buccal swabs, tissue homogenates, plasma, serum, and cultured cells. It operates on the principle of magnetic bead-based separation using Revvity’s proprietary polyvinyl alcohol (M-PVA) functionalized magnetic particles and electromagnetic separation technology—eliminating centrifugation, vacuum manifolds, or column-based binding steps. This approach ensures consistent binding kinetics, minimal shearing of high-molecular-weight nucleic acids, and robust recovery across variable input volumes (10 µL to 10 mL). Designed for deployment in clinical laboratories, biobanks, translational research cores, and molecular diagnostics facilities, the Chemagic 360 delivers reproducible yields with low inter-run CVs (<8% for DNA yield across 96 replicates), supporting downstream applications such as qPCR, NGS library preparation, Sanger sequencing, and HLA typing.
Key Features
- Electromagnetic separation module enabling precise, contactless bead manipulation—reducing mechanical wear and eliminating carryover risk between wells
- Three interchangeable extraction heads (low-, medium-, and high-volume configurations) optimized for 10 µL–10 mL sample inputs without manual reconfiguration
- On-deck spin-mixing mechanism ensuring homogeneous lysis and binding while preventing cross-contamination between adjacent wells
- Ambient-temperature operation throughout all protocol phases—minimizing aerosol generation and preserving labile RNA integrity
- Integrated Chemagic Auto Dispenser for accurate, multi-channel reagent delivery (including lysis buffers, wash solutions, and elution buffers) with programmable volume ranges (10–1000 µL)
- Modular architecture supporting seamless integration with third-party liquid handling platforms (e.g., Hamilton STAR, Tecan Freedom EVO) via standard RS-232/USB/OPC UA interfaces
Sample Compatibility & Compliance
The Chemagic 360 accommodates a broad spectrum of primary and processed biospecimens under standardized protocols compliant with ISO 20387:2018 (Biobanking), CLSI EP12-A2 (Evaluation of Precision), and ASTM D7492-22 (Standard Practice for Evaluation of Nucleic Acid Extraction Methods). Its open reagent architecture supports validated chemagen-branded kits (e.g., Chemagic Viral DNA/RNA Kit, Chemagic PBMC DNA Kit) as well as select third-party magnetic bead kits when used with appropriate protocol adaptation. All extraction workflows are fully traceable: each run logs operator ID, timestamp, barcode-scanned sample IDs, reagent lot numbers, and thermal/magnetic actuation parameters—meeting GLP/GMP documentation requirements and FDA 21 CFR Part 11 audit trail specifications.
Software & Data Management
Chemagic Software v5.x provides a Windows-based graphical interface with intuitive drag-and-drop protocol builder, real-time instrument status monitoring, and integrated barcode scanning (1D/2D). It supports LIMS interoperability through HL7 v2.5 and ASTM E1384-compliant data export formats (CSV, XML, PDF). Audit trails record every user action—including protocol edits, run initiation, and result exports—with immutable timestamps and digital signature support. Raw extraction logs include cycle-by-cycle magnetic field strength, mixing duration, incubation temperatures (ambient only), and fluid dispensing accuracy metrics—all archived locally and exportable for internal QA review or regulatory submission.
Applications
- High-throughput genomic DNA isolation from EDTA blood for biobanking and population genomics studies
- Routine viral RNA extraction from nasopharyngeal swabs and bronchoalveolar lavage fluid for respiratory pathogen surveillance
- Simultaneous co-extraction of host DNA and microbial RNA from tissue biopsies in infectious disease research
- HLA allele typing workflows requiring high-purity, inhibitor-free DNA from peripheral blood mononuclear cells (PBMCs)
- Pre-analytical processing for single-cell sequencing pipelines where input flexibility and low background contamination are critical
- Automated sample prep for companion diagnostics assays requiring CAP/CLIA-aligned validation documentation
FAQ
Is the Chemagic 360 compatible with non-chemagen magnetic bead kits?
Yes—subject to protocol optimization and analytical verification per ISO 15189 Annex B; Revvity provides technical guidance but does not validate third-party reagents.
What is the maximum allowable particulate load in lysates before processing?
Samples with >5% cellular debris or insoluble aggregates require pre-clearing by brief centrifugation (e.g., 10,000 × g, 2 min); the system does not include onboard filtration.
Can the Chemagic 360 be operated in a Class II biosafety cabinet?
Yes—the footprint (90 × 82 × 90 cm, L×W×H) and ambient-temperature operation allow safe installation within standard laminar flow hoods; electromagnetic interference is shielded per IEC 61326-1.
Does the system support remote monitoring or cloud-based data backup?
Local network logging is supported; cloud integration requires customer-configured secure file transfer (SFTP) or API middleware—not natively embedded.
How frequently must the electromagnetic separation module be calibrated?
Calibration is performed automatically at startup; annual verification by Revvity Field Service Engineers is recommended per ISO/IEC 17025 quality management guidelines.
