DLAB SK-D3309-Pro LCD Digital 3D Orbital Shaker

Overview

The DLAB SK-D3309-Pro LCD Digital 3D Orbital Shaker is an engineered platform for low-shear, uniform agitation in life science laboratories. Unlike conventional linear or orbital shakers, this instrument employs a true three-dimensional motion profile—combining horizontal orbital rotation with controlled vertical oscillation and a fixed 9° tilt angle—to generate gentle yet effective mixing across heterogeneous sample volumes. This kinematic design ensures consistent reagent penetration during gel staining and destaining, efficient washing of membranes in Western blotting and immunoprecipitation workflows, and homogeneous suspension of adherent or semi-adherent cells during incubation steps. Its compact footprint, IP21-rated enclosure, and wide-input voltage architecture (100–240 V, 50/60 Hz) enable reliable operation inside standard CO₂ incubators or ambient lab environments without thermal or electrical constraints.

Key Features

• True 3D orbital motion with precisely calibrated 9° tilt angle for optimized fluid dynamics and reduced sample stratification
• Dual independent LCD displays—one for real-time speed (10–70 rpm) and one for remaining timer duration (1 min to 19 h 59 min)
• DC motor drive delivering stable speed control with <±1 rpm repeatability under load (5 kg max, including clamps)
• RS232 serial interface enabling bidirectional communication with host PCs for remote parameter configuration and live speed/timer logging
• Free Shaker PC software supporting protocol storage, time-stamped operational logs, and exportable CSV data files compliant with internal GLP documentation requirements
• Incubator-compatible mechanical design: fanless operation, low electromagnetic emission, and non-corrosive housing materials suitable for humid, 5–40 °C environments
• Modular clamp system supporting universal microplate carriers (96-/384-well), tube racks (1.5–50 mL), and custom fixtures (sold separately)

Sample Compatibility & Compliance

The SK-D3309-Pro accommodates standard laboratory consumables including polypropylene and polystyrene microplates, conical centrifuge tubes, hybridization bottles, and blotting trays. Its 9° tilt geometry minimizes meniscus disruption and splashing during prolonged runs—critical for preserving antigen-antibody binding integrity in immunoassays and preventing gel cracking in nucleic acid electrophoresis post-staining. The device meets IEC 61010-1:2010 safety standards for laboratory equipment and carries CE marking for EMC and LVD compliance. While not temperature-controlled, its stable mechanical performance supports ISO/IEC 17025-aligned validation protocols when integrated into QC/QA workflows for molecular biology and biopharmaceutical process development.

Software & Data Management

The included Shaker PC software provides a validated interface for configuring run parameters—including ramp-up profiles, multi-step timed sequences, and speed hold intervals—and visualizing real-time operational status via dynamic waveform plots. All session metadata (start time, duration, setpoint, actual speed deviation) are automatically timestamped and stored locally with optional network backup. Audit trail functionality records user logins, parameter changes, and firmware updates—supporting FDA 21 CFR Part 11 readiness when deployed in regulated GMP environments. Exported datasets conform to ASTM E2500-22 guidelines for instrument data integrity and traceability.

Applications

• Gel electrophoresis: uniform Coomassie/silver staining and destaining of SDS-PAGE and agarose gels
• Immunoblotting: high-efficiency blocking, primary/secondary antibody incubation, and TBST wash cycles on PVDF or nitrocellulose membranes
• Molecular hybridization: stringent DNA/RNA probe binding in Southern/Northern blot procedures
• Cell-based assays: gentle resuspension of suspension cultures and maintenance of monolayer integrity during media exchange
• Protein purification: equilibration and elution buffer mixing in affinity chromatography workflows
• Diagnostic assay development: reproducible antigen-coating and conjugate incubation steps in ELISA platform optimization

FAQ

Can the SK-D3309-Pro operate inside a CO₂ incubator?

Yes—the unit is designed for incubator use with no active cooling fans, minimal heat dissipation (50 W total power draw), and materials rated for continuous exposure to 5–40 °C and ≤80% RH.
Is the RS232 interface compatible with modern Windows/macOS systems?

Yes; the interface requires a USB-to-RS232 adapter (standard FTDI chipset) and is supported by the Shaker PC software on Windows 10/11 and macOS 12+. Driver installation is plug-and-play.
What is the accuracy and stability of speed control across the full 10–70 rpm range?

Speed is maintained within ±1 rpm tolerance at all setpoints under nominal 5 kg load, verified per ISO 17025-accredited calibration procedures using optical tachometry.
Does the device support programmable multi-stage protocols?

Yes—the PC software enables creation of up to 10-step sequences with independent speed, duration, and pause settings per stage, stored as reusable method files.
Are replacement clamps and plate carriers available through DLAB’s global distribution network?

Yes—DLAB maintains certified accessory kits for 96-well plates, 50-mL conical tubes, hybridization bottles, and custom OEM fixtures, all traceable to lot-specific QC certificates.