SCIEX TripleTOF 5600+ High-Resolution Accurate-Mass QTOF Mass Spectrometer (Refurbished)

Overview

The SCIEX TripleTOF 5600+ is a high-resolution, accurate-mass quadrupole time-of-flight (QTOF) mass spectrometer engineered for comprehensive qualitative and quantitative analysis in complex matrices. Combining the robust ion transmission architecture of SCIEX triple quadrupole platforms with an accelerated orthogonal time-of-flight (TOF) mass analyzer, this system delivers both nominal and exact mass measurement in a single acquisition mode. Its hybrid design enables simultaneous high-sensitivity targeted quantitation—comparable to triple quadrupole performance—and untargeted, high-resolution discovery workflows. The platform is widely deployed in regulated pharmaceutical development (e.g., metabolite identification, impurity profiling), large-scale proteomics and metabolomics studies, and forensic or environmental unknown screening where mass accuracy ( 35,000 FWHM at m/z 1,000), and dynamic range (> 5 orders) are critical.

Key Features

• IonDrive™ Turbo V Source: Redesigned electrospray and APCI interface featuring optimized gas dynamics and heater geometry for enhanced stability at high flow rates (up to 1 mL/min), improved ion yield, and long-term reproducibility across multi-day runs.
• QJet® Ion Guide: Wide-bore RF-only ion funnel providing high-efficiency ion capture and transmission from atmospheric pressure to the first quadrupole, minimizing signal loss and improving duty cycle.
• High-Frequency LINAC® Collision Cell: Operates at elevated RF drive frequencies to maintain tight ion focus during fragmentation, enabling efficient MS/MS generation with high transmission and minimal energy spread—critical for SWATH® Acquisition consistency.
• Accelerator TOF™ Analyzer: Orthogonal acceleration combined with reflectron optics and high-voltage acceleration yields superior mass resolving power, mass accuracy stability, and sensitivity across wide m/z ranges (m/z 50–2,000).
• Dual-Mode Operation: Seamless switching between information-dependent acquisition (IDA) for discovery and SWATH® Acquisition for data-independent, comprehensive fragment ion coverage—each run generating fully digital, re-interrogatable datasets.
• FDA 21 CFR Part 11 Compliance Support: Audit trail, electronic signatures, user access controls, and secure data handling protocols enabled via SCIEX OS software with validated configuration.

Sample Compatibility & Compliance

The TripleTOF 5600+ demonstrates broad compatibility with liquid chromatography interfaces, including ESI, APCI, and nanoESI sources. It is routinely coupled with UHPLC systems such as the Shimadzu Prominence UFLC (included in this refurbished configuration), supporting gradient elution from 0.05–2.0 mL/min with column temperatures up to 80 °C. All hardware and firmware components meet IEC 61010-1 safety standards. Data integrity practices align with GLP/GMP expectations for method validation and reporting. The system supports ISO/IEC 17025-compliant instrument qualification documentation (IQ/OQ/PQ templates available upon request). No modifications have been made to original factory calibration or security architecture.

Software & Data Management

Controlled by SCIEX OS v2.4 (or later, included), the system provides unified acquisition, processing, and reporting environments. Key software modules include PeakView® for quantitative MRM and HRMS workflows, MasterView™ for spectral library management, and MarkerView™ for multivariate statistical analysis. SWATH® Acquisition data is processed using SpectraBridge™ and PeakView’s SWATH® MicroApp, enabling retrospective extraction of analytes without prior knowledge. Raw data files (.wiff) are stored in vendor-native format with embedded metadata; export to open formats (mzML, mzXML) is supported. All software installations are licensed, activated, and verified prior to shipment. Audit trails record user actions, parameter changes, and data processing steps in accordance with 21 CFR Part 11 requirements.

Applications

• Pharmaceutical R&D: Structural elucidation of drug metabolites, characterization of biologics (intact mass, deamidation, oxidation), and impurity identification per ICH Q5A/Q5D guidelines.
• Proteomics: Label-free and label-based (TMT, iTRAQ) quantitation, PTM mapping (phosphorylation, glycosylation), and top-down/bottom-up hybrid workflows.
• Metabolomics & Lipidomics: Untargeted profiling of polar and non-polar metabolites in biofluids and tissues; lipid class identification using retention time–mass correlation databases.
• Clinical & Regulatory Testing: Biomarker verification in plasma/serum, therapeutic drug monitoring (TDM), and residue analysis in food/environmental samples meeting EU SANTE/2023/12596 criteria.
• Forensic Toxicology: Broad-spectrum screening of novel psychoactive substances (NPS) and synthetic opioids with confirmatory MS/MS spectra at sub-ng/mL levels.

FAQ

Is this instrument fully refurbished and performance-verified?
Yes. This TripleTOF 5600+ underwent full functional testing at SpectraLab Scientific’s ISO 17025-accredited facility, including mass calibration, resolution verification, sensitivity assessment (using reserpine and Glu-Fibrinopeptide B), and system suitability checks against manufacturer specifications.
What LC system is included, and is it operational?
The configuration includes a complete Shimadzu Prominence UFLC system: CBM-20A Lite controller, CTO-20AC column oven, DGU-20A degasser, dual LC-20AD XR pumps, SIL-20AC XR autosampler, and associated tubing/fittings—all tested and calibrated.
Does the warranty cover both hardware and software support?
The 12-month comprehensive warranty covers all mechanical, electronic, and vacuum components. Remote software support and minor configuration assistance are included; major software upgrades or custom method development require separate service agreement.
Can this system be integrated into an existing LIMS or ELN environment?
Yes. SCIEX OS supports standard data export protocols (ODBC, CSV, XML) and offers API access for integration with third-party laboratory informatics systems. Validation documentation for LIMS interfacing is available on request.
Are consumables and spare parts available post-purchase?
All original SCIEX and Shimadzu consumables—including Turbo V source components, QJet guides, TOF detector assemblies, and UFLC pump seals—are stocked and available with lead times under 5 business days.