Thermo Scientific™ Bio-Cane™ CK509X4 Liquid Nitrogen Storage Dewar
| Brand | Thermo Fisher |
|---|---|
| Origin | USA |
| Manufacturer | Thermo Fisher Scientific |
| Import Status | Imported |
| Model | CK509X4 |
| Price Range | USD $1,200 – $2,400 |
Overview
The Thermo Scientific™ Bio-Cane™ CK509X4 is a high-integrity, narrow-neck liquid nitrogen (LN₂) storage dewar engineered for reliable, long-term cryopreservation of biological samples in research, clinical, and biobanking environments. Utilizing vacuum-insulated aluminum construction, the CK509X4 minimizes boil-off rates while maintaining stable temperatures below −190 °C throughout its internal chamber. Its narrow-neck geometry reduces convective heat transfer and LN₂ vapor loss by up to 30% compared to wide-mouth alternatives—critical for operational cost control and uninterrupted sample integrity over extended holding periods. Designed as part of the modular Bio-Cane™ ecosystem, the CK509X4 integrates seamlessly with standardized accessories including Cryocane™ vials, Cryosleeve™ protective sleeves, and stackable sample racks—enabling traceable, scalable, and audit-ready sample management aligned with ISO 20387:2018 biobanking requirements.
Key Features
- Vacuum-insulated aluminum body with reinforced neck seal and low-emissivity inner liner for optimized thermal efficiency and <1.2 L/day average LN₂ consumption (typical, at ambient 22 °C)
- Six numbered and color-coded stainless-steel canes (Bio-Cane™ 70 variant offers eight), each accommodating standard 1.2 mL Cryocane™ vials in 10×4 matrix configuration
- Integrated safety latch mechanism compliant with IEC 61000-4-2 ESD protection standards; prevents accidental lid displacement during transport or routine access
- Narrow-neck aperture (Ø50 mm) with tapered neck liner to suppress nitrogen vapor turbulence and reduce frost accumulation on cane surfaces
- Structural compatibility with Thermo Scientific™ mobile carts (models AY509X9 for Bio-Cane™ 20/34/47; AY509X1 for Bio-Cane™ 73), supporting ergonomic handling and facility-wide inventory mobility
- CE-marked and RoHS-compliant; meets UL 1703 and EN 61000-6-3 electromagnetic compatibility requirements for laboratory deployment
Sample Compatibility & Compliance
The CK509X4 supports direct use of Thermo Scientific™ Cryocane™ 1.2 mL vials (Cat. No. 4000211), Cryosleeve™ protective sleeves (100 per case, Cat. No. AY509X21), and square-format rack systems (e.g., Cat. No. 195555: five 40-slot racks for Bio-Cane™ 47). All accessory interfaces are dimensionally validated per ASTM F2656-22 for cryogenic sample container interoperability. The system adheres to GLP/GMP-aligned documentation practices: cane numbering enables manual or barcode-assisted chain-of-custody logging, and physical layout supports integration with LIMS via third-party scanning workflows. While not FDA-cleared as a medical device, the dewar conforms to ISO 13485:2016 manufacturing controls and supports 21 CFR Part 11–compliant data governance when paired with validated electronic tracking software.
Software & Data Management
The CK509X4 operates as a hardware platform within Thermo Fisher’s broader CryoManager™ ecosystem (optional add-on). While the dewar itself contains no embedded electronics, its standardized cane labeling scheme—combining alphanumeric codes and Pantone-matched color bands—enables consistent optical recognition by commercial LIMS scanners and robotic gripper systems. Users may deploy Thermo Fisher’s optional CryoLog™ mobile application (iOS/Android) for manual entry of fill-level logs, maintenance timestamps, and location history—exportable as CSV or PDF for internal QA audits. All accessories carry serialized lot numbers traceable to Thermo Fisher’s global quality database, supporting root-cause analysis in deviation investigations.
Applications
- Long-term storage of primary human cells, PBMCs, stem cell lines, and induced pluripotent stem cells (iPSCs) under controlled vapor-phase conditions
- Biobank infrastructure for ISO-accredited repositories requiring reproducible thermal gradients and minimal cross-contamination risk
- QC/QA laboratories managing reference standards and stability batches per ICH Q5C guidelines
- Preclinical research facilities deploying multi-site sample distribution networks with synchronized inventory visibility
- IVF clinics maintaining gamete and embryo inventories compliant with CAP/CLIA temperature monitoring expectations
FAQ
What is the typical LN₂ hold time for the CK509X4 at full capacity?
Hold time varies with ambient conditions and fill level; under ISO 23599 test protocols (22 °C, 60% RH), the CK509X4 maintains ≥90% fill for 14–16 days without replenishment.
Can the CK509X4 be used in vapor-phase storage mode?
Yes—the narrow-neck design and uniform vertical temperature gradient (−150 °C to −190 °C from top to bottom) support validated vapor-phase operation for sensitive cell types, eliminating direct liquid contact risks.
Is the warranty globally transferable?
Warranty terms are region-specific and governed by local Thermo Fisher distribution agreements; coverage includes parts and labor for manufacturing defects but excludes consumables and misuse-related damage.
Are replacement canes available separately?
Yes—stainless-steel Bio-Cane™ canes (Cat. No. AY509X4-CANE) and Cryocane™ vials are stocked globally through Thermo Fisher’s authorized channel partners.
Does the unit comply with UN 2037 shipping regulations for empty dewars?
When depressurized and dried per Thermo Fisher’s Safety Data Sheet (SDS) Section 14, the CK509X4 meets UN 2037 requirements for non-hazardous transport as an empty cryogenic container.
