Cole-Parmer FH100D Single-Channel Precision Peristaltic Pump

Overview

The Cole-Parmer FH100D is a single-channel, microprocessor-controlled peristaltic pump engineered for precision fluid handling in life science laboratories, bioprocessing workflows, and analytical instrumentation integration. Based on the fundamental principle of positive displacement via roller compression of flexible tubing, the FH100D delivers non-invasive, contamination-free transfer—ensuring that only the tubing contacts the fluid path. This design eliminates valves, seals, and dead volumes, enabling dry-run capability, self-priming, zero backflow, and compatibility with aggressive, shear-sensitive, or particulate-laden media—including biological suspensions, cell cultures, viscous polymers, and two-phase mixtures. Its compact footprint (12.5″ × 11″ × 6″) and robust architecture support deployment in biosafety cabinets, environmental chambers, and automated platforms where space, reliability, and regulatory traceability are critical.

Key Features

• High-accuracy flow control: ±0.25% repeatability when used with calibrated tubing and flow sensors—validated per ISO 5725-2 for precision and trueness.
• Multi-mode operation: Selectable manual knob control, analog voltage/current input (0–10 V / 4–20 mA), or digital command interface (TTL pulse or RS-485 Modbus RTU) for seamless PLC or SCADA integration.
• Programmable dispensing modes: Volume-based, time-based, and cyclic delivery protocols—each configurable with start/stop triggers, direction reversal (CW/CCW), and ramped acceleration profiles to minimize pulsation-induced stress on sensitive samples.
• Durable, service-friendly construction: Modular pump head with tool-free tube loading; interchangeable materials (e.g., GF nylon for chemical resistance, stainless steel for sterilizable applications) compliant with USP Class VI and FDA 21 CFR Part 11–ready audit trail logging when paired with compatible software.
• Low-maintenance operation: No lubrication, no valves, no seals—only periodic tubing replacement required. Compatible with silicone, Viton®, Pharmed® BPT, and Tygon® tubing sets certified for USP / cytotoxicity and extractables testing.

Sample Compatibility & Compliance

The FH100D supports a broad spectrum of fluid types without compromising integrity: Newtonian and non-Newtonian liquids (up to ~50,000 cP), gas-liquid slurries, low-conductivity solvents, and sterile biologics. Its non-contact pumping mechanism prevents cross-contamination between runs—essential for GLP-compliant sample preparation, HPLC mobile phase delivery, fermentation feed control, and cell therapy media formulation. All electrical components meet IEC 61000-6-2 (immunity) and IEC 61000-6-4 (emissions) standards. Mechanical safety complies with UL 61010-1 and EN 61010-1; CE marking confirms conformity with the EU Machinery Directive 2006/42/EC and Electromagnetic Compatibility Directive 2014/30/EU.

Software & Data Management

While the FH100D operates autonomously via front-panel controls, it interfaces with Cole-Parmer’s optional PumpMaster™ PC software (Windows-based) for full parameter logging, protocol archiving, and remote monitoring. The software supports timestamped event records—including speed changes, direction toggles, run duration, and error flags—for 21 CFR Part 11 compliance when configured with electronic signatures and role-based access control. Real-time analog output (4–20 mA or 0–10 V) provides closed-loop feedback to external controllers, while TTL tachometer pulses (5 V, 1 pulse/revolution) enable independent flow verification using external counters or data acquisition systems.

Applications

• Cell culture media perfusion and feeding in bioreactors and microfluidic organ-on-chip systems
• Automated reagent dispensing in ELISA, PCR setup, and clinical chemistry analyzers
• Continuous sampling for online UV-Vis or pH monitoring in fermentation tanks
• Low-shear transfer of fragile liposomes, exosomes, or protein aggregates
• Calibration fluid delivery in metrology labs adhering to ISO/IEC 17025 requirements
• Environmental water analysis—acid preservation, filtration bypass, and standard addition workflows

FAQ

Can the FH100D be integrated into a GMP manufacturing environment?
Yes—when deployed with validated tubing, documented calibration procedures, and PumpMaster™ software configured for audit trails and electronic signatures, the FH100D supports GMP-aligned process validation per Annex 11 and PIC/S guidelines.

Is tubing calibration required for volumetric accuracy?
For ±0.25% flow accuracy, tubing must be calibrated using gravimetric or timed-volume methods per ASTM D3417 or ISO 4787. Factory-calibrated tubing kits are available for common inner diameters (0.5–6.4 mm).

What is the maximum allowable ambient humidity for continuous operation?
The unit is rated for operation at ≤80% RH non-condensing, consistent with IP31 ingress protection. For high-humidity cleanrooms, optional conformal-coated PCBs are available upon request.

Does the pump support bidirectional communication over RS-485?
Yes—the FH100D implements Modbus RTU slave protocol (addressable up to 247 nodes), allowing readback of real-time RPM, direction status, fault codes, and cumulative runtime.

How often should tubing be replaced under continuous 24/7 operation?
Typical service life ranges from 100–500 hours depending on tubing material, pressure load, and rotational speed. Silicone tubing at 60 RPM typically lasts ~300 hours; Pharmed® BPT extends this to ~800 hours under identical conditions.