Supelco KLVIPSPE-12 / KLVIPSPE-24 Semi-Automatic Solid Phase Extraction System

Overview

The Supelco KLVIPSPE-12 and KLVIPSPE-24 are semi-automatic solid phase extraction (SPE) systems engineered for high-reproducibility sample preparation in regulated and research laboratories. Based on the fundamental principles of adsorption chromatography, these systems facilitate selective retention, washing, and elution of target analytes from complex matrices using disposable SPE cartridges. Unlike liquid–liquid extraction (LLE), SPE minimizes emulsion formation, reduces solvent consumption by up to 70%, and significantly improves analyte recovery—particularly for polar, thermally labile, or low-concentration compounds. Designed for compatibility with standard 1-mL to 10-mL SPE cartridges (including Supelco’s wide portfolio of bonded silica phases), the system supports method development and routine QC workflows across pharmaceutical, environmental, food safety, and clinical toxicology applications. Its modular architecture enables seamless integration into existing sample prep pipelines prior to HPLC, GC, LC-MS/MS, or ICP-MS analysis.

Key Features

• Patented spiral-flow control valve enabling precise, independent adjustment of vacuum-driven flow rates per channel—critical for consistent breakthrough prevention and optimal cartridge conditioning.
• Dual configuration options: 12-position (KLVIPSPE-12) and 24-position (KLVIPSPE-24) manifolds accommodate high-throughput labs without sacrificing reproducibility.
• Chemically inert fluid path: All solvent-contact surfaces constructed from PTFE-sealed valves, borosilicate glass reservoirs, and polypropylene collection racks—resistant to aggressive solvents including acetonitrile, methanol, dichloromethane, and 0.1 M NaOH.
• Cross-contamination mitigation design: Unique cartridge alignment geometry and isolated vacuum channels prevent carryover between adjacent positions during sequential processing.
• Ergonomic lid with integrated support feet allows stable, hands-free placement on benchtops during cartridge loading or fraction collection—reducing workflow interruption and operator fatigue.
• Vacuum interface compliant with standard 6.35-mm (¼-inch) vacuum tubing; compatible with laboratory-grade vacuum pumps (recommended ultimate pressure ≤ 50 mbar) and optional vacuum controllers for GLP-compliant pressure logging.

Sample Compatibility & Compliance

The KLVIPSPE platform processes diverse sample types—including serum, plasma, urine, milk, fruit juice, wastewater, soil extracts, tissue homogenates, and pharmaceutical formulations—without modification to hardware. It accommodates all major SPE cartridge formats (1 mL, 3 mL, 6 mL, and 12 mL volumes) and supports both positive-pressure (via nitrogen manifold) and vacuum-driven protocols. The system is routinely validated per ASTM D5907 (for water analysis), USP (chromatographic separations), and ISO 17025-accredited SOPs. When operated with audit-trail-capable vacuum controllers and electronic logbooks, it meets data integrity requirements under FDA 21 CFR Part 11 for regulated bioanalytical laboratories.

Software & Data Management

As a hardware-only platform, the KLVIPSPE series does not include embedded software. However, its standardized mechanical interface enables full traceability when paired with LIMS-integrated vacuum controllers (e.g., Vacuubrand VACUU·SELECT or Agilent Vacuum Manager). Operators document run parameters—including applied vacuum level, dwell times per step (conditioning, loading, washing, elution), and cartridge lot numbers—in paper-based or electronic batch records. For GxP environments, we recommend supplementing with validated electronic notebooks (e.g., LabArchives or Benchling) to maintain ALCOA+ compliance for raw data archiving and reviewer access logs.

Applications

• Clinical & forensic toxicology: Isolation of opioids, benzodiazepines, and stimulants from serum and urine prior to LC-MS/MS quantification.
• Environmental monitoring: Extraction of PAHs, PCBs, and organochlorine pesticides from groundwater and sediment extracts per EPA Method 353.1 and 8081B.
• Food safety testing: Cleanup of mycotoxins (aflatoxin B1, ochratoxin A) from cereal and nut matrices before HPLC-FLD detection.
• Pharmaceutical QC: Removal of excipients and degradation products from tablet dissolution samples prior to stability-indicating HPLC assays.
• Agrochemical residue analysis: Multi-residue SPE cleanup of fruits and vegetables prior to GC-ECD or LC-MS/MS screening per SANTE/11813/2017 guidelines.

FAQ

Is the KLVIPSPE system compatible with non-Supelco SPE cartridges?
Yes—mechanically compatible with all industry-standard 1–12 mL SPE cartridges featuring polypropylene housings and fritted discs.
Can the system be used under positive pressure instead of vacuum?
Yes—when coupled with a nitrogen or air pressure manifold (not included); requires optional pressure-regulating adapter kit.
What maintenance is required for long-term reliability?
Routine cleaning of PTFE valve seats with methanol and inspection of glass reservoir O-rings every 6 months; no calibration required.
Does Supelco provide method validation support for this platform?
Yes—application notes, certified reference method packages (e.g., EPA 525.3 for drinking water), and technical assistance are available through authorized distributors.
Are replacement parts such as glass reservoirs or valve assemblies available separately?
Yes—all consumable and wear components are stocked globally under Supelco part numbers KLVIPSPE-G12 (glass reservoir set) and KLVIPSPE-V12 (valve assembly kit).